IL-2, Human is a Oglycosylated, four α-helix bundle cytokine that has potent stimulatory activity for antigen-activated T cells.

Interleukin-2 (IL-2) is a Oglycosylated, four α-helix bundle cytokine that has potent stimulatory activity for antigen-activated T cells. It is expressed by CD4+ and CD8+ T cells, γδ T cells, B cells, dendritic cells, and eosinophils. IL-2/IL-2R signaling is required for T-cell proliferation and other fundamental functions which are essential for the immune response. IL-2 stimulates growth and differentiation of B-cells, NK cells, lymphokine activated killer cells, monocytes, macrophages and oligodendrocytes.

產品簡介

Species ：Human 

Protein Construction： IL-2 (Ala21-Thr153)   Accession # P60568 

 Purity ：> 95% as analyzed by SDS-PAGE 

Endotoxin Level： < 0.2 EU/μg of protein by gel clotting method 

Biological Activity ：ED50 < 2.0 ng/ml, measured in a cell proliferation assay using CTLL-2 cells. 

Expression System ：HEK 293 

Apparent Molecular Weight ：15~16 kDa, on SDS-PAGE under reducing conditions. 

Formulation ：Lyophilized after extensive dialysis against PBS. 

Reconstitution： It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH?O or PBS up to 100 μg/ml. 

Storage & Stability ：Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles. 

技術背景

Target Background ：Interleukin-2 (IL-2) is a Oglycosylated, four α-helix bundle cytokine that has potent stimulatory activity for antigen-activated T cells. It is expressed by CD4+ and CD8+ T cells, γδ T cells, B cells, dendritic cells, and eosinophils. IL-2/IL-2R signaling is required for T-cell proliferation and other fundamental functions which are essential for the immune response. IL-2 stimulates growth and differentiation of B-cells, NK cells, lymphokine activated killer cells, monocytes, macrophages and oligodendrocytes. 

Synonyms： IL2; IL-2; TCGF; lymphokine; interleukin 2 

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.